Specifications

MINI-PAM-II/B and MINI-PAM-II/R

Optoelectronic Unit
MINI-PAM-II/B (BLUE Version)

Measuring light: Blue (470 nm) LED, standard modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz and 100 Hz, measuring light PAR at standard settings = 0.05 μmol m-2 s-1. Fluorescence at wavelengths greater than 630 nm is measured

Actinic light: Same blue LED as for measuring light, maximum actinic PAR = 3000 μmol m-2 s-1, maximum PAR of saturation pulses = 6000 μmol m-2 s-1 adjustable at increments of 500 μmol m-2 s-1

 

Optoelectronic Unit
MINI-PAM-II/R (RED Version)

Measuring light: Red (655 nm) LED, modulation frequencies and PAR as described for MINI-PAM-II/B. Fluorescence at wavelengths greater than 700 nm is measured

Actinic light: Same red LED as for measuring light, maximum PAR of actinic light and saturation pulses as described for MINI-PAM-II/B

Optoelectronic Unit
MINI-PAM-II/B & MINI-PAM-II/R

Far red light: Peak emission at 735 nm

Signal detection: PIN photodiode protected by long-pass and a short-pass filters

Data memory: Flash memory, 8 MB, providing memory for more than 27000 saturation pulse analyses

Display: Backlit 160 x 104 dots (78 x 61 mm) transflective B/W LCD display with resistive touchscreen

Ports: Ports for fiberoptics, USB cable, external light source, 2035-B leaf clip, auxiliaries and 12 V DC power supply

Power supply: 6 AA (Mignon) rechargeable batteries (Eneloop 1.2 V/2 Ah), providing power for up to 1000 yield measurements; 6 spare batteries, automatic power/off, battery charger (100 to 240 V AC, 50-60 Hz, 0.35 A) for 1 to 8 AA/AAA NI-MH/NI-CD batteries, 12 V 5.5 A power supply MINI PAM-II/N

Operating temperature: -5 to +45 °C (non-condensing)

Dimensions: 17.2 cm x 11.2 cm x 7.6 cm (L x W x H)

Weight: 1.5 kg (incl. batteries)

 

Fiberoptics MINI-PAM/F

Design: Randomized 70 μm glass fibers forming single plastic shielded bundle with stainless steel adapter ends

Dimensions: Active diameter 5.5 mm, outer diameter 8 mm, length 100 cm

Weight: 180 g

Power Supply MINI-PAM-II/N

Input: 100 to 240 V AC, 50 to 60 Hz

Output: 12 V DC, 5.5 A

Operating temperature: -5 to +45 °C (non-condensing)

Dimensions: 13 cm x 5.5 cm x 3 cm (L x W x H)

Weight: 350 g including cables

 

Battery Charger 000190101101

Input: 100 to 240 V AC, 50 to 60 Hz

Output: 12 V DC, 1.0 A

Operating temperature: -5 to +45 °C (non-condensing)

Dimensions: 17.5 cm x 10.5 cm x 3 cm (L x W x H)

Weight: 300 g including cable

Distance Clip 60° 2010-A

Design: Metal clip with fiber holder and 11 mm sample hole: 5.5 cm x 1.4 cm (L x W)

Complementary Items

Sloped Plexiglas rack for convenient desktop operation. Stylus for touchscreen. Carrying strap for optoelectronic unit

Transport Case MINI-PAM-II/T

Design: Aluminum case with custom foam packing

Dimensions: 50 cm x 34 cm x 20 cm (L x W x H)

Weight: 3.8 kg

 

Software WinControl-3

Program: WinControl-3 System Control and Data Acquisition Program (Microsoft Windows XP/Vista/7/8/10) for operation of measuring system via PC, data acquisition and analysis

Saturation Pulse Analysis: Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. PAR, leaf temperature and relative humidity using 2035-B Leaf-Clip Holder. Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines: Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features: Automatic determination of signal offset for all light intensities and all gain levels. Automatic calibration of internal PAR sensor against an external PAR sensor connected to the MINI-PAM-II

Communication Protocol: USB

Computer Requirements: Processor, 0.8 GHz. RAM, 512 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 2.0/3.0

ACCESSORIES

Accessories

MINI-PAM-II: Standard Configuration

Miniature Spectrometer MINI-SPEC/MP

The Miniature Spectrometer MINI-SPEC/MP records spectra of PAR in the visible and far red range. The MINI-SPEC/MP is, thus, well-suited to study effects of spectral variations of light on photosynthesis. The spectrometer can also be configured to measure sample reflectance which can be used as a proxy for the sample’s spectral absorption properties. Another configuration permits measurements of fluorescence emission spectra of samples where fluorescence excitation occurs either with blue or with green light. A reflectance standard and a special 160 cm cable connecting the MINI-SPEC/MP to the MINI-PAM-II are part of delivery.

Light, Temperature and Humidity Sensing Leaf Clip Holder 2035-B

The leaf clip 2035-B has been devised to record leaf temperature, light intensities at sample level and air humidity. The clip measures photosynthetically active radiation (PAR) by an LS-C Mini Quantum Sensor, a NiCr-Ni thermocouple records leaf temperature, and a capacitive type, temperature-corrected humidity sensor measures relative humidity of air. Calibration factors of PAR and temperature sensors are stored on the internal memory of the leaf clip 2035-B. A second PAR sensor can be connected to the leaf clip. Measured data are sent as digital signals to the MINI-PAM-II. To facilitate studies under field conditions, fluorescence measurements can be triggered by pushing the control button of the leaf clip 2035-B.

External LED Light Source 2054-L

The 2054-L external light source complements the internal actinic light of MINI-PAM-II fluorometers. The 2054-L can be easily attached to the 2035-B leaf clip. The external lamp provides light peaking at 630 nm (red), 520 nm (green), and 452 nm (blue), as well as white light. Each of the four channels has a standard maximum photon flux density of 1500 µmol m-2 s-1; the composition of colors is freely selectable. The light source is connected to the SYNC port of the MINI-PAM-II fluorometer and it is controlled by the MINI-PAM-II or by a computer running the WinControl-3 software.

2035-B/RLC Conversion Kit for 2030-B/2035-B Leaf Clips

The 2035-B/RLC kit shields the sample area of 2030-B/2035-B Leaf Clips from external light. The accessory has been designed for field experiments in which photosynthesis shall be driven exclusively by the fluorometer’s internal light source. The 2035-B/RLC kit is especially suitable for rapid light curve (RLC) experiments in outdoor studies.

Fiberoptics Holder for Surfaces 2060-A

The holder positions the fiberoptics of the MINI-PAM-II on bulky samples. Combinable with the 2065-M Mini Quantum/Temp.-Sensor, to measure temperature and impinging PAR of the surface area investigated.

Dark Leaf Clip DLC-8

The DLC-8 leaf clip permits dark-acclimation of small leaf areas in the field. The tip of the fiberoptics of the MINI-PAM-II fits exactly in the DLC-8 port. With the fiber tip inserted, the sliding shutter of the DLC-8 can be opened so that F0 and FM level fluorescence can be measured without interference of ambient light.

Arabidopsis Leaf Clip 2060-B

Aluminum clip with small measuring area designed to position small leaves below the fiberoptics of the MINI-PAM-II. When combined with the 2065-M Mini Quantum/Temp.-Sensor, PAR on sample level and lower leaf temperature is recorded.

Suspension Cuvette KS-2500

The suspension cuvette includes a 400 μl sample compartment made of stainless steel with PVC exterior. The cuvette is equipped with a 7 mm fiberoptics window adapter, an injection port for Hamilton syringes, and nozzles for connecting an external flow-through water-bath for temperature control.

Mini Quantum/Temp.-Sensor 2065-M

Precise mini quantum and temperature sensors usable independently or in conjunction with the 2060-B Arabidopsis Leaf Clip or the 2060-A Fiberoptics Holder for Surfaces.

Magnetic Stirrer with Fiberoptics
Holder MKS-2500

The device is equipped with a specially modified stirrer plate to center and hold the KS-2500 Suspension Cuvette. The MKS-2500 Magnetic Stirrer comes with a Perspex base plate with stand bar for mounting fiberoptics on top of cuvette.

Leaf Positioning Setup DUAL-BA

The DUAL-BA has been designed to easily and quickly position the fiber optics of a PAM fluorometer on attached leaves. The accessory is compatible with the DUAL-PAM/F, MINI-PAM-II and PAM-2500 fluorometers. It includes a duct with laterally mounted neodymium magnet and a screw to lock the fiber optics tip. A fixation angle made of spring steel strip positions the leaf in front of the fiber optics, whereby the steel strip is hold by the magnet of the duct. The DUAL-BA includes a fiber optics guide with metal rod to mount the guide on a stand.

Miniature Fiberoptics MINI-PAM/F1

The MINI-PAM/F1 is useful when small surfaces are to be investigated. It consists of a single coated plastic fiber which provides an active diameter of 2 mm.

Barcode Scanner BCS-9590

The barcode scanner is the ideal add-on when many differed samples are repeatedly probed. Simply mark your samples by barcodes. Then, the BCS-9590 scanner writes for each saturation pulse analysis the sample ID into the memory of the MINI-PAM-II or the report data in WinControl-3. The barcode scanner is connected to the COMP 1 or COMP 2 port of the MINI-PAM-II.

Compact Tripod ST-2101A

The tripod serves for positioning of the Leaf Clip Holder 2035-B, the Mini Quantum/Temp.-Sensor 2065-M, or the Arabidopsis Leaf Clip 2060-B.

Fiberoptics Adapter 90º 2030-B90

The fiberoptics adapter 90º can be attached to the leaf clips 2035-B and 2060-B to position the fiberoptics af the MINI-PAM-II at 90° angle relative to leaf plane.

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