WATER-PAM-II

Chlorophyll Fluorometer for Phytoplankton

The WATER-PAM-II is next to PHYTO-PAM-II, the instrument of choice for phytoplankton analysis. It can be used for laboratory cultures as well as natural water samples when very low chlorophyll contents of samples require ultra-sensitive fluorescence detection.

The WATER-PAM-II is a portable cuvette system for analysing the photosynthetic activity of a wide variety of phytoplankton samples. The light weight instrument offers a wide range of applications thanks to its large sensitivity spectrum, the ability to easily switch between red or blue light mode (measuring light and actinic light) and a variety of accessories such as a flow-through cuvette.

Specifications

WATER-PAM-II

Optoelectronic Unit WATER-PAM-II

Measuring light: Blue (450 nm) red (630 nm) LED, standard modulation frequencies 5 to 25 Hz, adjustable in increments of 5 Hz, and 100 Hz, measuring light PAR at standard settings = 0.05 µmol m-2 s-1. For differential algae analysis auxiliary LEDs 520 and
660 nm.

Actinic light: Blue (450 nm) and red (630) LED as for measuring light, maximum actinic
PAR = 3000 µmol m-2 s-1, maximum PAR of saturation pulses = 6000 µmol m-2 s-1 adjustable at increments of 500 µmol m-2 s-1 expandable on request to
12000 µmol m-2 s-1 adjustable at increments of
1000 µmol m-2 s-1

Far red light: Peak emission at 730 nm

Signal detection: Hamamatsu photomultiplier protected by long-pass and a short-pass filters

Cuvette holder: for 15 mm Ø cuvette, Perspex inlet for overspill protection, POM cuvette cover 35 mm high x 40 mm Ø

Data memory: Flash memory, 8 MB, providing memory for more than 27,000 saturation pulse analyses

Display: Backlit 160×104 dots (78 x 61 mm) transflective B/W LCD display with resistive touchscreen

Ports: PAR sensor, Temp, AUX1, AUX2, SYNC, Stirrer, CTRL1, CTRL2, COMP, EXT DC and USB

Power supply: 8 AA (Mignon) rechargeable batteries (Eneloop 1.2 V/2 Ah), providing power for up to 1000 yield measurements; 8 spare batteries, automatic power/off, battery charger (100 to 240 V AC, 50-60 Hz, 0.35 A) for 1 to 8 AA NI-MH batteries, 12 V 5,5 A power supply MINI PAM-II/N

Operating temperature: -5 to +45 °C, (non-condensing)

Dimensions: 17.2 cm x 13.5 cm x 7.6 cm (L x W x H)

Weight: 1.5 kg (incl. battery)

 

Power Supply MINI-PAM-II/N

Input: 100 to 240 V AC, 50 to 60 Hz

Output: 12 V DC, 5.5 A

Operating temperature: -5 to +45 °C, (non-condensing)

Dimensions: 13 cm x 5.5 cm x 3 cm (L x W x H)

Weight: 350 g including cables

Battery Charger 000190101101

Input: 100 to 240 V AC, 50 to 60 Hz

Output: 12 V DC, 1.0 A

Operating temperature: -5 to +45 °C, (non-condensing)

Dimensions: 17.5 cm x 10.5 cm x 3 cm (L x W x H)

Weight: 300 g including cable

 

Software WinControl-3

Program: WinControl-3 System Control and Data Acquisition Program (Windows 8 und 10, 32-bit and 64-bit; Linux on request) for operation of measuring system via PC, data acquisition and analysis

Saturation Pulse Analysis: Measured: Ft, F0, FM, F, F0‘ (also calculated), FM‘. PAR, Calculated: F0‘ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines: Differential algae analysis; two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features: light curve protocols; induction curve protocols; Actinic+Yield protocols; clock and batch file operation; automatic determination of signal offset and background fluorescence (originated e.g. by yellow substances) for all light intensities and all gain levels; automatic calibration of internal PAR using light sensor US-SQS/L

Communication Protocol: USB

Computer Requirements: Minimum screen resolution 1280 x 800 pixel, USB interface, Windows operating system or Linux on request. Interface, USB 2.0/3.0

Transport Case WATER-PAM/T

Design: Aluminum case with custom foam packing

Dimensions: 50 cm x 34 cm x 20 cm (L x W x H)

Weight: 3.8 kg

 

Accessories

 

Stirrer WATER-II/S

Design: Instrument operated stirrer with disposable perspex stirring paddles. Scope of delivery includes a set (10 pieces) of Stirring Paddles WATER-R.

Dimensions: 71 mm x 30 mm (l x Ø)

Weight: 95 g

Stirring Paddles WATER-R

Design: Set of 10 perspex stirring paddle for WATER-II/S

 

Flow-Through Cuvette PHYTO-II/FT

Design: Cuvette holder made from black polyoxymethylene polymer (POM) with in-/out water tubing connectors (4 mm inner diameter; 6 mm outer diameter) The cuvette is mounted with two knurled screws to the optical unit of the instrument (PHYTO-PAM-II COMPACT Version or WATER-PAM-II). Delivery includes a POM mounting device for the Spherical Micro Quantum Sensor US-SQS.

Dimensions: : Ø 49 mm, with connectors and cuvette 64 mm x 74 mm

Weight: 150 g

Barcode Scanner BCS-9590

Design: Single-line handheld laser scanner with trigger button and 1 m, partially coiled cord; to be connected to the Photosynthesis Yield Analyzer MINI-PAM-II or WATER-PAM-II. Bar codes are stored together with fluorescence data

Dimensions: 9 cm (max.) x 6 cm (max.) x 16 cm (max.) (L x W x H)

Weight: 335 g

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